Method of treating animals infected with large roundworms using cobalt arsenic compounds



United States Patent 3,466,363 METHOD OF TREATING ANIMALS INFECTED WITH LARGE ROUNDWORMS USING COBALT ARSENIC COMPOUNDS Orley J. Mayfield, Charles City, Iowa, assignor, by mesne assignments, to Kewanee Oil Company, Bryn Mawr, Pa., a corporation of Delaware No Drawing. Filed July 23, 1965, Ser. No. 474,491 Int. Cl. A61k 25/00 US. Cl. 424-295 1 Claim ABSTRACT OF THE DISCLOSURE This invention comprises a composition suitable for any method of treating animals infected with large roundworms comprising a feed composition containing water and cobalt arsenate, cobalt arsanilate, or cobalt phenylarsonate.

This invention relates to the prevention of roundworms, especially large roundworms in domestic animals and poultry, such as chickens, turkeys, etc. By preventing the large roundworms, I am able to prevent diseases which are caused by parasites on the large roundworms. By prevention of roundworms, I mean that they are subjected to a dosage of a therapeutic agent at a plurality of intervals and in amounts sufficient to kill a sufficient number, or all of such roundworms so that the infestation of the animal or poultry is avoided, cured, or controlled.

The therapeutic agent which I prefer to use for preventing roundworm infestation is cobalt arsanilate. I may also use any of the cobalt salts of phenylarsonic acids, preferably those which are substituted on the ring by a halogen group, a methyl group, a phenyl group, a chlorophenyl group, a nitro group, a hydroxy group, a ureido group, or a plurality of such groups and mixtures thereof, and cobalt arsinoxide. Also included is cobalt arsenate.

The parasites against which said cobalt compounds set forth above are effective are those known as large roundworms, such as the Ascaridia, especially Ascaridia galli, and the Heterakidae, especially heterakis gallinae.

The cobalt arsanilate, although milder to the domestic animal and more palatable, at the same concentration than, for example, the sodium salt, is no less effective. Indeed it is more effective for preventing large roundworms than the corresponding sodium salt.

Arsanilic acid and its sodium salt have been suggested heretofore but not the cobalt salt. On the organic side the art has grown toward the phenylarsonates. On the metal side, sodium salts have been preferred while cobalt is little noticed. So far as applicant is aware, nobody has previously used cobalt arsanilate as a coccidosis control through roundworm control.

The quantity of cobalt arsanilate to be used can vary with respect to the weight of the basic feed compositions. The concentration of cobalt arsanilate should be such that when the bird eats the basic food ration ad libitum, it will receive the correct dosage of the cobalt arsanilate. In general, poultry should receive a dosage in the range of from one-fourth of a pound to one pound per ton of dry solid feed or an equivalent amount if supplied by way of edible oil, or other feed, or water. These equivalent dosages can be calculated easily by the formulator and indicated suitably on the containers.

It is an object of the present invention to provide a novel and eifective therapeutic treatment for large roundworms in poultry.

3,466,363 Patented Sept. 9, 1969 ice It is another object to provide a proper dosage of cobalt for obtaining freedom from or partial freedom from the parasite without damage to the poultry being treated.

Another object is to produce a therapeutic composition having prophylactic properties relative to poultry roundworm disease and which is adapted for oral ingestion with feedstuif or drinking water.

A still further object is to provide a medicament for roundworm control which is palatable to poultry.

Other objects of the invention will become obvious as the description proceeds, it being understood that this description is not limiting as to the scope of the invention.

In place of cobalt arsanilate, arsanilic acid and cobalt sulfate may be used together. These compounds may be mixed and then added to the feed or di'inking water. It is, however, not necessary to mix the cobalt and the arsanilic acid. It is enough if both are added and eventually mixed with the feed. Obviously, it is preferable to mix the feed and additives at the time the feed is compounded. This should give a uniform mix and reaction willtake place upon ingestion of the mixture, if not before.

The following examples will serve to illustrate the invention:

EXAMPLE I This experiment was conducted to determine the value of cobalt arsenate and cobalt arsanilate in the prevention of roundworm (Ascaridia) infestation when administered at the /2 pound per ton level in feed. 75-day-old chicks were randomized by the card shuflie method, weighed, wingbanded, and divided into three groups of 25 chicks each. Group I was given untreated feed and held as a control; group II was given feed otherwise the same but containing cobalt arsanilate at the above-mentioned level; group HI was given feed containing cobalt arsenate at the above-mentioned level. All chicks were given embryonated Ascaridia ova on the first, seventh, and fourteenth day of the test. The total number of worms per group was determined by postmortem examination at the end of the fourth week. There were 14 worms present in group I; 8 worms present in group II; and no worms present in group III. Hence, the efficacy of cobalt arsanilate was nearly 50 percent, while that for cobalt arsenate was 100 percent.

GROUP I Weighing dates and wts. in grams Ascaridia Bird No PM exaan, 5/3 5/10 5/17 5/24 5/31 N o. worms 295 36 76 136 253 401 300 35 56 100 205 334 961 35 75 120 208 312 964 36 75 145 255 357 976- 33 126 221 354 32 60 120 225 354 0 33 61 130 235 372 34 55 122 223 341 38 76 138 236 364 37 78 140 239 392 33 80 143 255 384 37 58 112 214 340 38 68 138 263 420 34 70 128 209 322 40 72 132 235 360 38 61 123 238 374 32 63 125 236 384 35 75 125 234 360 39 152 270 424 39 76 125 233 356 35 51 208 328 32 63 220 344 35 71 133 230 360 39 62 105 135 290 37 Died 5/8/ navel infection Avg. wt 35. 68 68. 22 127. 20 228. 74 359. 87

PNOOOOQQOOOOOHHHHOOO HON The total feed consumption for the above group for the time period of the test was 34 pounds 9 ounces.

GROUP II Weighting dates and wts. in grams Ascaridia PM exam., Bird No. 5/3 5/10 5/17 5/24 5/31 No. worms Total wt 934 1, 829 3, 321 5, 901 9, 102 8 Avg. wt 37. 36 73. 16 132. 84 236.0 364.08

The total feed consumption for the above group for the time period of the test was 36 pounds 12 ounces.

GR 0 UP III Weighting dates and wts. in grams Asearidia PM exam., /3 5/10 5/17 5/24 5/31 No. worms 39 60 118 210 339 0 40 68 116 188 300 0 35 65 116 185 298 0 35 67 112 190 300 0 36 72 128 208 327 0 35 68 126 212 321 0 35 64 115 192 308 0 38 65 116 202 331 0 35 49 90 158 269 0 36 61 113 200 312 0 40 64 115 185 294 0 33 58 116 206 228 0 35 48 100 180 294 0 34 52 107 185 305 0 39 68 125 222 258 0 35 52 105 165 145 0 37 68 126 205 323 0 38 66 124 212 338 0 35 65 124 212 338 0 35 16 136 223 345 0 35 Died 5/4 navel infection 49 62 108 195 283 0 35 68 125 210 328 0 35 70 125 210 333 0 42 72 130 218 338 0 Total win... 812 1, 528 2, 810 4, 747 7, 317 0 Avg. wt 36.48 63.66 117.0 137.79 304.87

The total feed consumption for the above group for the time period of the test was 34 pounds.

EXAMPLE II 75-day-old chicks were randomized by the card shufiie method, weighed, Wingbanded, and divided into three groups of 25 chicks each. One group was given untreated feed and held as a control; another group was fed the same food to which had been added cobalt arsenate in the ratio of 4 lb, per ton of feed; a third group was given the same food containing cobalt arsanilate in the ratio of /2 lb. per ton of food. All chicks were given embryonated Ascaridia galli (roundworm) at the beginning of the test, and again a week after it started. The birds receiving oobalt arsenate contained 39 worms compared with in the control, equivalent to 40% efficacy. The birds treated with cobalt arsanilate contained 16 worms compared with 65in the control, or efficacy.

EXAMPLE III This experiment was conducted to determine the value of cobalt arsanilate in preventing infestation with roundworms in comparison to sodium arsanilate. One hundredday-old chicks were randomized by the card shuflle method, wingbanded, and weighed, and placed in four groups of 25 each. Group 1 was a control on regular feed; group 2 received feed containing cobalt arsanilate at the rate of 4 lb. per ton; group 3 received feed containing cobalt arsanilate at the level of 1 lb. per ton; group 4 received feed containing sodium arsanilate at 1 lb. per ton. The birds were given embryonated ascarida at one day and seven days of age. The dosage of ascaridia ova was too heavy (approximately 400) at one day of age, and killed several of the birds. The dosage was reduced to approximately 100 ova per chick on the seventh day. The test was discontinued at six weeks. Postmortem examination showed 72 roundworms in the control group, 51 worms in the group receiving /1 lb. per ton of cobalt arsanilate, 14' roundworms in the group receiving feed containing 1 lb. per ton cobalt arsanilate, and group 4 showed 125 roundworms. The eflicacy of cobalt arsanilate at 4 lb. per ton was 40%; at 1 lb. per ton, while that of sodium arsanilate was zero. Evidently, the normal resistance of the bird was undermined in the case of group 4.

EXAMPLE 1V Cobalt arsanilate (growth test) Purpose: To determine the value of cobalt arsanilate as a growth stimulant when administered at 45, 90, and 180 grams per ton of feed.

Procedure: 100-day-old chicks were weighed, wingbanded, and randomized by the card shuffie method into four groups.

JMedication: Birds were given the following medication:

Group I1.68 grams cobalt arsanilate in 75 lb. feed (45 gram ton) Group II3.37 grams cobalt arsanilate in 75 lb. feed gram ton) Group III6.74 grams cobalt arsanilate in 75 lb. feed .(180 gram ton) Group IVstraight feed, 75 lbs.

All birds were fed the following feed formula:

Yellow corn meal "pounds" 200 44% soybean meal do 70 Fish meal do 15 Alfalfa meal do 15 Bone meal do 3 Limestone do 6 Iodized salt do 2 Vitamin A, LU. 800,000 Vitamin D I.U. 175,000 Vitamin B mg 180 Niacin mg 2,200 Riboflavin mg 480 Pantothenic ..mg 450 Choline mg 24 Vitamin B mg 5 Vitamin E, I.U,

Conclusion:

Group I with 45 grams per ton made a 2% gain over the controls.

Group II with 90 grams per ton made a l /2% gain over the controls.

Group III with 180 grams per ton made a 6% gain over the controls.

GROUP I Weighing dates and weights in grams Total wt 10,077 2, 304 4, 284 7,409 11, 542 15, 111

Avgwt 40. 92.16 171. 36 296.36 461.48 604.64

The total food consumption for the above group for the time period of the test was 77 pounds.

GROUP II Weighing dates and weights in grams 10/18 10/25 11/1 11/8 11/15 11/22 35 72 143 252 405 522 34 72 150 263 422 444 40 80 145 246 388 493 40 90 170 292 468 628 40 Died 10/23 wry-neck, cecal core 40 92 176 305 475 635 39 80 160 270 425 558 37 82 165 275 438 568 41 88 165 282 444 610 41 92 170 282 424 535 44 100 196 335 514 645 42 96 195 340 510 662 42 93 194 333 518 653 40 90 162 273 422 562 40 90 170 300 484 446 39 90 175 305 578 705 37 95 183 315 492 645 39 100 180 298 488 670 34 73 135 230 360 465 35 92 173 296 472 610 38 96 180 310 485 632 37 Died 10/22 starvation 43 108 205 345 562 755 38 95 182 312 486 615 40 90 166 287 454 585 Total Wt 975 2, 062 5, 941 6, 747 10, 654 13, 843

Avgwt 39.00 89. 65 171. 34 293. 34 426 601. 86

The total food consumption or the above group for the time period of the test was 75 pounds.

GROUP III Weighing dates and weights in grams Avg. wt 39. 76 92. 32 117. 88 296.8 470 626. 40

The total food consumption for the above group for the time period of the test was 79 pounds.

GROUP IV Weighing dates and weights in grams Avg. Wt 39. 28 90. 12 170. 76 393. 04 461 594. 28

The total food consumption for the above group for the time period of the test was 79 pounds.

EXAMPLE V Purpose: To determine the value of Cobalt Arsanilate in the prevention of roundworm (Ascaridia galli), cecal worms (Heterakis) and blackhead.

Procedure: 79-day-o1d turkey poults were received May 9, 1964, Wingbanded, weighed, and divided into four groups.

Medication:

Group I-Straight feed control 100 lbs.

Group II11.3 grams cobalt Arsanilate in 100 lbs. feed. Group III16.9 grams cobalt arsanilate in 100 lbs. feed. Group IV-22.7 grams cobalt arsanilate in 100 lbs. feed Feed formula:

Infection: All turkeys given embryonated Ascaridia galli and Heterakis at 7 and 14 days of age.

The control group made a gain of 24 lbs. on 68 /2 lbs. of feed or 1 lb. gain to 2.8 lbs. feed.

Group II-% lb. cobalt arsanilate per ton gained 29 lbs. on 66 lbs. feed or 1 lb. gain to 2.6 lbs. feed or 20% increase in weight.

Group III----% lb. cobalt arsanilate per ton gained 34 lbs. on 77 lbs. feed or 1 lb. gain to 2.2 lbs. feed or 41% increase in weight.

Group IV.1 1b. cobalt arsanilate per ton gained 35 lbs. on 88 lbs. feed or 1 lb. gain to 2.4 lbs. feed or 45% increase in weight.

GROUP I Weighing dates and weights in grams 85 108 205 343 514 719 76 104 182 297 455 667 94 126 210 319 510 740 76 82 Died 5/25 Blackhead 78 134 230 379 596 865 105 165 286 420 512 872 84 122 176 273 422 648 80 107 170 263 391 556 75 118 218 343 534 788 72 96 155 242 388 604 Died 5/22 Enteritis 90 132 210 329 594 724 88 108 162 278 435 650 82 122 211 326 488 78 112 172 263 418 682 84 Died 5/19 decomposed 88 151 248 342 451 590 96 155 254 394 608 919 84 140 240 325 458 605 84 132 230 374 556 806 Avg 50 90 81. 65 204.11 317. 35 480. 47 701. 35

The total ieed consumption for the above group for the time period of the test was 76% pounds.

GROUP 11 Weighing dates and weights in grams 51 90 252 414 596 830 54 88 152 285 480 727 1, 020 53 Died 5/13 Sac Infection 56 95 166 305 510 74 1, 024 59 86 230 352 514 706 52 62 92 156 262 411 603 53 82 108 194 327 530 780 54 78 122 196 333 560 660 50 75 8 262 392 587 862 48 Died 5/12 of Navel Infection 54 95 1 265 453 640 905 54 93 156 255 361 550 805 52 74 95 301 468 671 47 68 102 145 218 338 510 52 86 146 260 419 663 934 44 66 110 309 485 710 53 55 99 126 198 318 512 52 86 125 194 316 469 680 53 66 104 298 480 725 48 95 162 282 491 728 1, 034

Avg 5 70 80.00 122. 7 219 357. 44 545. 00 787. 27

The total feed consumption for the above group for the time period of the test was 76 pounds.

GROUP III Weighing dates and weights in grams Avg 105 162 266 430 670 970 108 183 315 507 739 1, 032 82 135 242 381 573 810 78 124 284 431 629 93 142 245 375 568 795 85 142 188 315 527 782 96 168 285 505 757 1, 065 92 156 285 484 747 1, 058 96 155 266 415 657 906 85 155 275 474 738 1, 011 82 140 218 330 518 775 105 182 312 527 807 1, 130 98 172 300 517 778 825 96 168 Died injury 85 125 20 2 769 The total feed consumption for the above group for the time period of the test was 76% pounds.

GROUP IV Weighing dates and weights in grams Bird Avg

The total feed consumption for the above group for the time period of the test was 88 pounds.

EXAMPLE VI This experiment was conducted to determine the value the same feed until October 24, then taken off medication and slaughtered on the 30th. During the last month, the hogs on the higher dosage of cobalt arsanilate gained weight at a considerably reduced rate, and at the time of slaughtering were 13 lbs. lighter than the controls. Those in group II at the end of the growth period had outgained the controls by 8 lbs. per pig. Group I gained 1 lb. per 3.1 lbs. of food; group II gained 1 lb. per 2.7 lbs. of food; while the control group gained 1 lb. per 3.2 lbs. of food. On postmortem examination 46 Ascaris were found in group HI, 40 in group II, and zero in group I. The greater dosage thus showed 100% complete roundworm control, while great stimulation was shown at the intermediate level.

EXAMPLE VII.GROWTH STIMULATION CHICKENS Day old Week 1 Week2 Week3 Week4 Week! Weekfi gain gain Cobalt arsenate:

ontrol 45 gmJton-treatedun 90 gm./ton-treated gm. /ton-treated Control L Elton-treated.

Cobalt arsanilate:

Control GROWTH STIMULATION The hogs in all three groups were fed embryonated Ascaris eggs July 1 and 7. All pigs were vaccinated with Pasteurella Corynebacterium bacterin and erysipelas bacterin on July 1. On August 1, the males were castrated and all vaccinated with blood origin-inactivated hog 70 Example I shows a partial kill of roundworms using the cobalt salt of arsanilic acid at one-half pound per ton of feed. This is a rather small concentration of the therapeutic agent. Example II is also an experiment at one-half pound per ton but substitutes cobalt arsenate. Here again a partial kill of the roundworms is seen at one-half pound per ton for cobalt arsanilate and one-fourth pound per ton for cobalt arsenate. Example III shows a gain in kill as the concentration goes up to one pound per ton. The

sodium arsanilate proved worthless. Example IV shows the results from increasing the dosage from 45 to 180 grams per ton. Here the growth promotion is reported and is shown to be satisfactory. Example V shows a control of 2.8 pounds feed to one pound gain in weight. At one-half pound cobalt arsanilate, this value dropped to 2.6, a distinct gain over the control. At three-fourths pound per ton, the figure goes down to 2.2. At one pound per ton of cobalt arsanilate, a value of 2.4 was obtained. Example VI shows the result in the case of pigs.

Example VII shows a considerable study of growth stimulation over a period of six weeks. In many instances, it will be noted, significant weight increases were obtained but in other instances, such increases were not significant. Control of roundworms was eifective, nevertheless, and why weight gains were sometimes obtained and sometimes were not is not fully understood. It appears, however, if cobalt arsenate is used rather than cobalt arsanilate, that weight gain is less likely.

What is claimed is:

1. A method for treating animals having roundworms which comprises orally administering to said animals the composition of a carrier selected from the group consisting of feed and water and containing from about 0.25 to 1.00 lbs. per ton of said carrier of a compound selected from the group consisting of cobalt arsenate, cobalt arsanilate and cobalt phenyl arsonatc.

References Cited Veter Bulletin, 33 pp. 204-205 (1963).

Organic Arsenical Compounds, Raiziss et al., pp. 240-243 and 257-259 (1923).

Poster: The Yearbook of Agriculture, pp. 83-84 (1950).

ALBERT T. MEYERS, Primary Examiner STANLEY I. FRIEDMAN, Assistant Examiner 

